Volume 1, Issue 3, August 2017, Page: 69-76
The Wastewater Nutrient Removal Efficiences of Chlorella sorokiniana and Scenedesmus obtusiusculus
Bryant Isaac Mbir, Department of Environmental Science, University of Cape Coast, Cape Coast, Ghana
Appah John Kwame Mensah, Department of Environmental Science, University of Cape Coast, Cape Coast, Ghana
Received: Apr. 21, 2017;       Accepted: May 27, 2017;       Published: Jul. 3, 2017
DOI: 10.11648/j.be.20170103.12      View  1784      Downloads  108
Urine treatment and nutrient removal was studied on a pilot scale in the DESAH building for a period of 3 months. The essence of the study was to evaluate the practical nutrient removal efficiencies of Chlorella sorokiniana and Scenedesmus obtusiusculus. The microalgae were grown on 3 different media― namely; mixture (mixed treated and untreated urine), untreated urine and control, and their nutrient removal efficiencies were investigated. Urine that has passed through the OLAND RBC system served as treated urine, and Bold’s basal medium served as the control. The OLAND RBC system was able to remove 95.7% of total chemical oxygen demand (COD), 27.1% total nitrogen, 99.7% ammonium, 88.6% total phosphorus and 89.3% ortho-phosphate from the influent urine. Low nutrient removal performance at a very high N: P molar ratios were observed in microalgae in the untreated urine. However, the nutrient removal capacities of microalgae were very high at reduced N: P molar ratios in the mixed medium. Chlorella sorokiniana was able to remove 63.2% TN and 55.8% TP at a low N: P molar ratio of 8.5:1, while Scenedesmus obtusiusculus removed 45.9% TN and 76.3% TP at an N: P molar ratio of 6.9:1. The results indicate that nutrient removal by microalgae is most efficient in mixed OLAND RBC treated and untreated urine culture. Therefore, the integration of the OLAND RBC system when designing microalgae induced wastewater treatment technologies for sanitation purposes is advocated.
Urine Treatment, Nutrient Removal, Microalgae Cultivation, Domestic Wastewater
To cite this article
Bryant Isaac Mbir, Appah John Kwame Mensah, The Wastewater Nutrient Removal Efficiences of Chlorella sorokiniana and Scenedesmus obtusiusculus, Bioprocess Engineering. Vol. 1, No. 3, 2017, pp. 69-76. doi: 10.11648/j.be.20170103.12
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Zeeman, G., Kujawa-Roeleveld, K. (2011). Resource recovery from source separated domestic waste (water) streams; full scale results. Accepted Manuscript to IWA - Accessed 7th January, 2011.
Kujawa-Roeleveld, K. and Zeeman, G. (2006). Anaerobic Treatment in Decentralised and Source-Separation-Based Sanitation Concepts, Reviews In Environmental Science and Bio/Technology 5, 115–139.
Hanæus, J., Hellström, and Johansson, E. (1997). A Study of Urine Separation System in an Ecological Village in Northern Sweden. Water Science and Technology 35 (9), 153-160.
Niwagaba, C., Nalubega, M., Vinneras, B., Sundberg, C. and Jonsson, H. (2009). Bench Scale Composting Of Source-Separated Human Faeces for Sanitation. Waste Management 29, 585-589.
Meinzinger, F., Oldenburg, M. and Otterpohl, R. (2009). No waste, but a resource: Alternative approaches to urban sanitation in Ethiopia. Desalination, 248, 322-329.
Zeeman, G. and Lettinga, G. (1999). The Role of Anaerobic Digestion of Domestic Sewage In Closing The Water And Nutrient Cycle at Community Level. Water Science and Technology 39 (5), 187-194.
Rounsefell, B. D. (2010). Laboratory-Scale Investigation Of The Decentralised Anaerobic Co- Digestion Of Blackwater And Food Waste For A Tourism Facility. Phd Thesis, School Of Civil Engineering, The University Of Queensland.
Wang, H., Xiong, H., Hui, Z. and Zeng, X. (2011). Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresource Technolgy, Article in press.
de-Bashan, L. E., Trejo, A., Huss, V. A. R., Hernandez, J. P. and Bashan, Y. (2008). Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater. Bioresour. Technol. 99: 4980 - 4989.
Bjornsson, W. J., Nicol, R. W., Dickinson, K. E., & McGinn, P. J. (2013). Anaerobic digestates are useful nutrient sources for microalgae cultivation: functional coupling of energy and biomass production. Journal of applied phycology, 25: 1523 - 1528.
Jia, H. and Yuan Q. (2016). Removal of nitrogen from wastewater using microalgae and microalgae–bacteria consortia. Cogent Environmental Science, 2: 1275089.
Griffiths, E. W. (2009). Removal and Utilization of Wastewater Nutrients for Microalgae Biomass and Biofuels, All Graduate Theses and Dissertations, Utah State University, Paper 631.
Tuantet, K., Janssen, M., Temmink, H., Zeeman, G., Wijffels, R. H. and Buisman, C. J. (2014). Microalgae growth on concentrated human urine. J. Appl. Phycol. 26: 287 - 297.
Zhang, J., Giannis, A., Chang, V. W., Ng, B. J. and Wang, J.-Y. (2013). Adaptation of urine source separation in tropical cities: process optimization and odor mitigation. J. Air Waste Manag. 63: 472 - 481.
STOWA (2005). DESAR, Options for separate treatment of urine. Rapport 11. Accessed 25th August, 2012.
Windey, K., Bo, I. D. and Verstraete, W. (2005). Oxygen-Limited autotrophic nitrification-Denitrification (Oland) In A Rotating Biological Contactor Treating High-Salinity Wastewatwer. Water Research 39, 4512-4520.
Kunikane, S., Kaneko, M. and Maehara, R. (1984). Growth and nutrient uptake of green alga, Scenedesmus dimorphus, under a wide range of nitrogen/phosphorus ratio—I. Experimental study. Water Research, 18 (10), 1299-1311.
Bohutskyi, P., Liu, K., Nasr, L. K., Byers, N., Rosenberg, J. N., Oyler, G. A., Betenbaugh, J. M. and Bouwer, E. J. (2015). Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate. Appl Microbiol Biotechnol. DOI 10.1007/s00253-015-6603-4.
Matusiak, K., Przytocka-Jusiak, M., Leszczyńska-Gerula, K., & Horoch, M. (1976). Studies on the purification of wastewater from the nitrogen fertillizer industry by intensive algal cultures. II. Removal of nitrogen from the wastewater. Acta Microbiologica Polonica, 25: 361.
Guštin, S. and Marinšek-Logar, R. (2011). Effect of pH, temperature and air flow rate on the continuous ammonia stripping of the anaerobic digestion effluent. Process safety and environmental protection, 89: 61 - 66.
Konig, A., Pearson, H., Silva, S. A., 1987. Ammonia toxicity to algal growth in waste stabilization ponds. Water Sci. Technol. 19: 115 - 122.
Wilsenach, J. A. (2006). Treatment of source separated urine and its effects on wastewater Systems. PhD Thesis, Delft University of Technology, The Netherlands.
Nurdogan, Y. and Oswald, W. (1995). Enhanced nutrient removal in high-rate ponds. Water Sci Technol 31: 33–43.
Eyster, C. (1978). Nutrient concentration requirements for Chlorella sorokiniana. Ohio J. Sci. 78: 79 - 81.
Udert, K. M., Fux, C., Münster, M., Larsen, T. A., Siegrist, H., & Gujer, W. (2003). Nitrification and autotrophic denitrification of source-separated urine. Water Science and Technology, 48: 119 - 130.
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