Volume 1, Issue 4, November 2017, Page: 93-103
Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3
Mani Kalaiyarasi, International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Kanyakumari District, Tamilnadu, India
Ponnuswamy Vijayaraghavan, International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Kanyakumari District, Tamilnadu, India
Subhanandharaj Russalamma Flanet Raj, Department of Zoology, Nesamony Memorial Christian College, Kanyakumari District, Tamilnadu, India
Samuel Gnana Prakash Vincent, International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Kanyakumari District, Tamilnadu, India
Received: May 6, 2017;       Accepted: Jun. 12, 2017;       Published: Jul. 31, 2017
DOI: 10.11648/j.be.20170104.11      View  1789      Downloads  121
A cheap agro-industrial waste was used as the substrate for the production of protease and cellulase from Bacillus cereus KA3. The process parameters were optimized by a two level full factorial design and response surface methodology. Two level full factorial designs revealed that the factors namely, pH, peptone and NaH2PO4 were significantly influenced on the production of protease and cellulase. These three significant factors were selected for central composite design and response surface methodology. The maximum protease and cellulase production was 3127 U/g, and 482 U/g, respectively, after statistical approach, which showed over fourfold increase in enzyme production than unoptimized medium.
Solid State Fermentation, Agro-residues, Response Surface Methodology, Optimization
To cite this article
Mani Kalaiyarasi, Ponnuswamy Vijayaraghavan, Subhanandharaj Russalamma Flanet Raj, Samuel Gnana Prakash Vincent, Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3, Bioprocess Engineering. Vol. 1, No. 4, 2017, pp. 93-103. doi: 10.11648/j.be.20170104.11
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Puri, S., Beg, Q. K, and Gupta, R. 2002, “Optimization of alkaline protease production from Bacillus sp. by response surface methodology,” Current Microbiology, vol. 44, pp. 286-290.
Azura, O. A. T., Abubakar, L. K., Hamid, F, Radu, N. S. A, Nazamid, S. S. 2009, “Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase- producing bacterium isolated from a Malaysian hotspring,” World Journal of Microbiology and Biotechnology, vol. 25, pp. 1981–1988.
Bhat, M. K. 2000. “Cellulases and related enzymes in biotechnology,” Biotechnology Advances, vol. 18, no. 5, pp. 355–383.
Sánchez, O. J., Cardona, C. A. 2008, “Trends in biotechnological production of fuel ethanol from different feedstocks,” Bioresource Technology, vol. 99, no. 13, 5270–5295.
Mawadza, C., Boogerd, F. C, Zvauya, R., Van Verseveld, H. W. 1996, “Influence of environmental factors on endo-β-1, 4-glucanase production by Bacillus HR68, isolated from a Zimbabwean hot spring,” Antonie van Leeuwenhoek, vol. 69, no. 4, pp. 363–369.
Rastogi, G., Muppidi, G. L, Gurram, R. N. 2009, “Isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the Homestake gold mine, Lead, South Dakota, USA,” Journal of Industrial Microbiology and Biotechnology, vol. 36, no. 4, pp. 585–598.
Prakasham, R. S., Rao, C. H. S, Sarma, P. N. 2006, “Green gram husk, an inexpensive substrate for alkaline protease production by Bacillus sp. in solid state fermentation.,” Bioresource Technology, vol. 97, pp. 449–454.
Olivera, A. N., Oliveira, L. A. Andrade, J. S. 2010, “Production and Some Properties of Crude Alkaline Proteases of Indigenous Central Amazonian Rhizobia Strains,” Brazilian Archives of Biology and Technology, vol. 53, no. 5, pp. 1185-1195.
Mekala, N. K., Singhania, R. P, Sukumaran, R. K. 2008, “Cellulase production under solid-state fermentation by Trichoderma reesei RUT C30: Statistical optimaization of process parameters”, Applied Biochemistry and Biotechnology, vol. 151, pp. 122-128.
Reddy, L. V. A., Wee, Y, Yun, J, Ryu, H. 2008, “Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett- Burman and response surface methodological approaches,” Bioresource Technology, vol. 99, pp. 2242-2249.
Liu, J, Xing, J, Chang, T, Ma, Z, Liu, H. 2005, “Optimization of nutritional conditions for natto kinase production by Bacillus natto NLSSE using statistical experimental methods,” Process Biochemistry, vol. 40, pp. 2757–2762.
Vijayaraghavan, P, Vincent S. G. P, 2014, “Medium optimization for the production of fibrinolytic enzyme by Paenibacillus sp. IND8 using response surface methodology,” The Scientific World Journal, vol. 2014, Article ID 276942, 9 pages, 2014.
Chen, W., Zhao, Z, Chen, S.-F, Li, Y. Q. 2008, “ Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro,” Bioresource Technology, vol. 99, pp. 3187 – 3194.
Ariffin, H. Abdullah, N. Umi Kalsom, M. S Shirai, Y, Hassan, M. A, 2006, “Production and characterisation of cellulase by bacillus pumilus eb3”, International Journal of Engineering and Technology, vol. 3, no. 1, pp. 47-53.
Sneath, P. H. A., Mair, N. S, Sharpe, M. E, Holt, J. E. (ed) 1986. Bergey”s manual of systemic bacteriology, Williams and Wilkins, Baltimore.
Lane, D. J, Pace, B, Olsen, G. J, Stahl, D. A, Sogin, M. L, Pace, N. R. 1985, “Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses,” Proceedings of National Academy Sciences USA, vol. 82, pp. 6955–6959.
Chopra, A. K. Mathur, D. K. 1985, “Purification and characterization of heat-stable protease from Bacillus stearothermophilus RM-67,” Journal of Dairy Sciences, vol. 68, pp. 3202-3211.
Irfan, M, Safdar, A, Syed, Q, Nadeem, M. 2012, “Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity,” Turkish Journal of Biochemistry, vol. 37, pp. 287–293.
Chauhan, B, Gupta, R. 2004, “Application of statistical experimental design for optimization of alkaline protease production from Bacillus sp. RGR-14,” Process Biochemistry, vol. 39, pp. 2115-2122.
Gautam, R., Hsu, N. C, Lau, K. M. 2010, “Premonsoon Aerosol Characterization and Radiative Effects over the Indo-Gangetic Plains: Implications for Regional Climate Warming,” Journal of Geophysical Research, vol. 115: D17208, doi: 10.1029/2 010JD013819.
Vonothini, G, Murugan, M, Sivakumar, K, Sudha, S, 2008, “Optimization of protease production by an actinomycete Strain, PS- 18A isolated from an estuarine shrimp pond,” African Journal of Biotechnology, vol. 7, no. 18, pp. 3225- 3230.
Manivasagan, P, Gnanam, S, Sivakumar, K, Thangaradjou, T, Vijayalakshmi, S, Balasubramanian, T, 2010, “Isolation, identification and characterization of multiple enzyme producing actinobacteria from sediment samples of Kodiyakarai coast, the Bay of Bengal,” African Journal of Microbiological Research, vol. 4, no. 14, pp. 1550-1559.
Bakare, A. A., Mosuro, A. A, Osibanjo, O, 2005, “An in vivo evaluation of induction of abnormal sperm morphology in mice by landfill leachates,” Mutation Research, vol. 582, pp. 28–34.
Chennupati, S, Potumarthi, R, Gopal Rao, M, Manga, P. L, Sridevi, M, Jetty, A, 2009, “Multiple responses optimization and modeling of lipase production by Rhodotorula mucilaginosa MTCC-8737 using response surface methodology,” Applied Biochemistry and Biotechnology, vol. 159, pp. 317–29.
Daniel, J. D, Silvana, T. S, Plinho, F. H, Brandelli, A, 2008, “Production of extracellular β‐glucosidase by Monascus pupureus on different growth substrates,” Process Biochemistry, vol. 42, no. 5), pp. 904‐908.
Bhaskar, N., Sudeepa, E. S, Rashmi, H. N, Tamil Selvi, A, 2007, “Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities,” Bioresource Technology, vol. 98, pp. 2758-2764.
Folasade, M. O., Joshua, O. A, 2008, “Some properties of extracellular protease from Bacillus licheniformis lbbl-11 isolated from “iru”, a traditionally fermented African locust bean,” Global Journal of Biotechnology and Biochemistry, vol. 3, pp. 42-46.
Pandey, A., Soccol, C. R, Nigam, P, Brand, D, Mohan, R, Roussos, S, 2000, “Biotechnological potential of coffee pulp and coffee husk for bioprocesses,” Biochemical Engineering Journal, vol. 6, pp. 153-162.
Potumarthi, R., Jacques, L, Harry, W, Michael, D, 2012, “Surface immobilization of Rhizopus oryzae (ATCC 96382) for enhanced production of lipase enzyme by multiple responses optimization,” Asia-Pacific Journal of Chemical Engineering, vol. 7, pp. S285–S295.
Kshetri, P., Ningombam, O, Ningombam, D. S, 2016, “Optimization of Alkaline Protease Production by Alkaliphilic Bacillussp. KW2 in Low Cost Medium using Statistical Approaches,” Applied Microbiology Open Access, vol. 2, pp. 1000117.
Mustafa, S. R., Ahmad, H, Nolasco, H. C., Hasnain, H, Nurshikin, S, Azman, R. H, 2016, “Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavus NSH9 under Solid State Fermentation,” Brazilian Archives of Biology and Technology, vol. 59, pp. 16150632.
Irfan, M., Mushtaq, Q, Tabssum, F, Shakir, H. A, Qazi, J. I, 2017, “Carboxymethyl cellulase production optimization from newly isolated thermophilic Bacillus subtilis K-18 for saccharification using response surface methodology,” AMB Express, vol. 7, pp. 29.
Vijayaraghavan, P., Arun, A, Prakash Vincent, S. G, Valanarasu, M, Aldhabi, N. A., 2016, “Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis,” Frontier in Microbiology, vol. 7, pp. 361.
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