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Optimization of Lactic Acid Fermentation from Overriped Plantain Using Response Surface Methodology (RSM)

Received: 30 November 2022     Accepted: 23 December 2022     Published: 24 March 2023
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Abstract

Lactic acid is a building block chemical used by many manufacturing industries. The search for cheap biochemical feedstock and process is of great concern to lactic acid producing industries. Plantain is a rich source of carbohydrate but the overriped plantains are thrown away as waste because of their non-firmness. This study evaluates the utilization of overripe plantain as biochemical feedstock for optimum production of lactic acid. Optimization of lactic acid production with overriped plantain was studied using Response Surface Methodology. The initial pH and reducing sugar content of the plantain hydrolysate were 4.89 and 166.05 g/l respectively. The response of lactic acid concentration to four factors: substrate concentration (138.25 – 166.05 g/L), initial pH (4 – 8), fermentation temperature (30 – 50°C) and time (24 – 168 h) was studied. The lactic acid concentration ranged from 123.50 – 163.00 g/l. A statistically significant [(Pmodel>F) < 0.0001] second order quadratic polynomial regression model was obtained for lactic acid production; the R2 and adjusted-R2 were 0.9935 and 0.9896 respectively. Numerical optimization was used to obtain optimum lactic acid production (157.53 g/ L) at glucose concentration, pH, temperature and time of 159.10 g/l, 7.0, 42.3°C and 60 h respectively. Overriped plantain was a good biochemical feedstock for lactic acid production.

Published in Bioprocess Engineering (Volume 7, Issue 1)
DOI 10.11648/j.be.20230701.11
Page(s) 1-9
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Lactic Acid, Optimization, Overripe Plantain, Fermentation, Response Surface Methodology

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    Alao Adeyinka Idowu, Agarry Samuel Enahoro, Afolabi Tinuade Jolaade. (2023). Optimization of Lactic Acid Fermentation from Overriped Plantain Using Response Surface Methodology (RSM). Bioprocess Engineering, 7(1), 1-9. https://doi.org/10.11648/j.be.20230701.11

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    Alao Adeyinka Idowu; Agarry Samuel Enahoro; Afolabi Tinuade Jolaade. Optimization of Lactic Acid Fermentation from Overriped Plantain Using Response Surface Methodology (RSM). Bioprocess Eng. 2023, 7(1), 1-9. doi: 10.11648/j.be.20230701.11

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    AMA Style

    Alao Adeyinka Idowu, Agarry Samuel Enahoro, Afolabi Tinuade Jolaade. Optimization of Lactic Acid Fermentation from Overriped Plantain Using Response Surface Methodology (RSM). Bioprocess Eng. 2023;7(1):1-9. doi: 10.11648/j.be.20230701.11

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  • @article{10.11648/j.be.20230701.11,
      author = {Alao Adeyinka Idowu and Agarry Samuel Enahoro and Afolabi Tinuade Jolaade},
      title = {Optimization of Lactic Acid Fermentation from Overriped Plantain Using Response Surface Methodology (RSM)},
      journal = {Bioprocess Engineering},
      volume = {7},
      number = {1},
      pages = {1-9},
      doi = {10.11648/j.be.20230701.11},
      url = {https://doi.org/10.11648/j.be.20230701.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20230701.11},
      abstract = {Lactic acid is a building block chemical used by many manufacturing industries. The search for cheap biochemical feedstock and process is of great concern to lactic acid producing industries. Plantain is a rich source of carbohydrate but the overriped plantains are thrown away as waste because of their non-firmness. This study evaluates the utilization of overripe plantain as biochemical feedstock for optimum production of lactic acid. Optimization of lactic acid production with overriped plantain was studied using Response Surface Methodology. The initial pH and reducing sugar content of the plantain hydrolysate were 4.89 and 166.05 g/l respectively. The response of lactic acid concentration to four factors: substrate concentration (138.25 – 166.05 g/L), initial pH (4 – 8), fermentation temperature (30 – 50°C) and time (24 – 168 h) was studied. The lactic acid concentration ranged from 123.50 – 163.00 g/l. A statistically significant [(Pmodel>F) R2 and adjusted-R2 were 0.9935 and 0.9896 respectively. Numerical optimization was used to obtain optimum lactic acid production (157.53 g/ L) at glucose concentration, pH, temperature and time of 159.10 g/l, 7.0, 42.3°C and 60 h respectively. Overriped plantain was a good biochemical feedstock for lactic acid production.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Optimization of Lactic Acid Fermentation from Overriped Plantain Using Response Surface Methodology (RSM)
    AU  - Alao Adeyinka Idowu
    AU  - Agarry Samuel Enahoro
    AU  - Afolabi Tinuade Jolaade
    Y1  - 2023/03/24
    PY  - 2023
    N1  - https://doi.org/10.11648/j.be.20230701.11
    DO  - 10.11648/j.be.20230701.11
    T2  - Bioprocess Engineering
    JF  - Bioprocess Engineering
    JO  - Bioprocess Engineering
    SP  - 1
    EP  - 9
    PB  - Science Publishing Group
    SN  - 2578-8701
    UR  - https://doi.org/10.11648/j.be.20230701.11
    AB  - Lactic acid is a building block chemical used by many manufacturing industries. The search for cheap biochemical feedstock and process is of great concern to lactic acid producing industries. Plantain is a rich source of carbohydrate but the overriped plantains are thrown away as waste because of their non-firmness. This study evaluates the utilization of overripe plantain as biochemical feedstock for optimum production of lactic acid. Optimization of lactic acid production with overriped plantain was studied using Response Surface Methodology. The initial pH and reducing sugar content of the plantain hydrolysate were 4.89 and 166.05 g/l respectively. The response of lactic acid concentration to four factors: substrate concentration (138.25 – 166.05 g/L), initial pH (4 – 8), fermentation temperature (30 – 50°C) and time (24 – 168 h) was studied. The lactic acid concentration ranged from 123.50 – 163.00 g/l. A statistically significant [(Pmodel>F) R2 and adjusted-R2 were 0.9935 and 0.9896 respectively. Numerical optimization was used to obtain optimum lactic acid production (157.53 g/ L) at glucose concentration, pH, temperature and time of 159.10 g/l, 7.0, 42.3°C and 60 h respectively. Overriped plantain was a good biochemical feedstock for lactic acid production.
    VL  - 7
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

  • Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

  • Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

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